Alternative Names:

MKK7; Jnkk2; MAPKK7; PRKMK7; JNK-activating kinase 2; MAPKK 7; Mitogen Activated Protein Kinase kinase 7; SAPKK4; stress-activated protein kinase kinase 4;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:40 FC 1:50

	Western blot analysis of MEK5 expression in Hela cell lysate.

Purification:

Affinity-chromatography

Specificity:

MEK7 Antibody detects endogenous levels of MEK7

Immunogen:

A synthesized peptide derived from human MEK7

Description:

MKK7 is strongly activated by TNF-α, as well as other environmental stresses, whereas SEK1/MKK4, which activates both p38 and SAPK/JNK pathways, is not activated by TNF-α. Sequence alignment of the activation loop of the MAP kinase kinase family members indicates that Ser271 and Thr275 are potential phosphorylation sites that are crucial for the kinase acivity.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.