Alternative Names:

Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K1; MEK-1; PRKMK1;

Application:

WB

Reactivity:

Human

Dilution:

WB 1:500~1:1000

	Western blot analysis of MEK5 expression in (1) HeLa cell lysate; (2) HeLa cell treated with Nocodazole.

Purification:

Affinity-chromatography

Specificity:

Phospho-MEK1 (T292) Antibody detects endogenous levels of Phospho-MEK1 (T292)

Immunogen:

A synthesized peptide derived from human MEK1

Description:

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation. Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.