Alternative Names:

5'-AMP-activated protein kinase catalytic subunit alpha-1; AAPK1; AMP-activate kinase alpha 1 subunit; AMP-activated protein kinase; AMPK; AMPK alpha 1; AMPK subunit alpha-1; PRKAA 1; ACACA kinase;

Application:

WB ICC/IF IP

Reactivity:

Human

Dilution:

WB 1:1000~1:2000 ICC/IF 1:50~1:200 IP 1:50

	Western blot analysis of Phospho-AMPK alpha 1 (S496) expression in (1) 293T cell lysate treated with LP; (2) 293T cell lysate.

Purification:

Affinity-chromatography

Specificity:

Phospho-AMPK alpha 1 (S496) Antibody detects endogenous levels of Phospho-AMPK alpha 1 (S496)

Immunogen:

A synthesized peptide derived from human AMPK alpha 1

Description:

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis. Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.