Alternative Names:

C-Raf; CRAF; Protein kinase raf 1; RAF1; Raf proto-oncogene serine/threonine-protein kinase; kinase Raf1;

Application:

WB IHC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:1000 IHC 1:50~1:100

	Western blot analysis of Phospho-Raf1 (S259) expression in (1) 293T cell lysate; (2) 293T cell lysates treated with Okadaic Acid + Calyculin A.

Purification:

Affinity-chromatography

Specificity:

Phospho-Raf1 (S259) Antibody detects endogenous levels of total Phospho-Raf1 (S259)

Immunogen:

A synthesized peptide derived from human Phospho-Raf1 (S259)

Description:

Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.