Alternative Names:

GXP1; MGC4781; POC20; GXP1; SMAX2; UBA1; UBE1;

Application:

WB IHC ICC/IF FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:1000~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:200 FC 1:20

	Western blot analysis of UBA1 expression in Jurkat cell lysate.

Purification:

Affinity-chromatography

Specificity:

UBA1 Antibody detects endogenous levels of total UBA1

Immunogen:

A synthesized peptide derived from human UBA1

Description:

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.