Alternative Names:

28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 27 kDa protein; HSP 27; HSP25; HSPB1; P25; SRP27; Stress-responsive protein 27;

Application:

WB IHC ICC/IF IP

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

	Western blot analysis of Phospho-Hsp27 (S78) expression in (1) A431 cell lysate; (2) A431 cell lysate treated with Anisomycin.

Purification:

Affinity-chromatography

Specificity:

Phospho-Hsp27 (S78) Antibody detects endogenous levels of total Phospho-Hsp27 (S78)

Immunogen:

A synthesized peptide derived from human Phospho-Hsp27 (S78)

Description:

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.