Alternative Names:

B-RAF; B-RAF proto-oncogene serine/threonine-protein kinase; BRAF1; RAFB1; RMIL; RMIL serine/threonine-protein kinase; c-RMIL; kinase B-Raf;

Application:

WB ICC/IF IP

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:30

	Western blot analysis of Phospho-B Raf (T401) expression in (1) PC-12 cell lysate; (2) PC-12 cell lysate treated with TPA.

Purification:

Affinity-chromatography

Specificity:

Phospho-B Raf (T401) Antibody detects endogenous levels of total Phospho-B Raf (T401)

Immunogen:

A synthesized peptide derived from human Phospho-B Raf (T401)

Description:

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.