Alternative Names:

FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A;

Application:

WB IHC ICC/IF FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:100~1:500 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50

	Western blot analysis of Ubiquitin expression in Jurkat cell lysate.
	Immunohistochemical analysis of paraffin-embedded human endometrium carcinoma, using K48-linkage Specific Ubiquitin Antibody.
	Immunofluorescent analysis of MCF-7 cells, using K48-linkage Specific Ubiquitin Antibody.

Purification:

Affinity-chromatography

Specificity:

K48-linkage Specific Ubiquitin Antibody detects endogenous levels of total K48-linkage Specific Ubiquitin

Immunogen:

A synthesized peptide derived from human K48-linkage Specific Ubiquitin

Description:

Plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.