Alternative Names:

EZH 2; EZH1; EZH2; EZH2b; KMT 6; KMT6; KMT6A;

Application:

WB IHC ICC/IF FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:1000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1：50

	Western blot analysis of KMT6 / EZH2 expression in HEK293 cell lysate.
	Immunohistochemical analysis of paraffin-embedded human tonsil, using KMT6 / EZH2 Antibody.
	Immunofluorescent analysis of Hela cells, using KMT6 / EZH2 Antibody.

Purification:

Affinity-chromatography

Specificity:

KMT6 / EZH2 Antibody detects endogenous levels of total KMT6 / EZH2

Immunogen:

A synthesized peptide derived from human KMT6 / EZH2

Description:

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.