Alternative Names:

4EBP1, EIF4EBP1, Eukaryotic translation initiation factor 4E binding protein 1, Insulin-stimulated EIF-4E binding protein PHAS-I, P/OKCL.6, PHAS-1, PHAS-I

Application:

WB IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200

	Western blot analysis of 4E-BP1 phosphorylation expression in EGF treated MDA-MB-435 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-4E-BP1 (Thr36) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Threonine 36

Immunogen:

A synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Threonine 36

Description:

4E-BP1 binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.