Alternative Names:

ADD2; ADDB; adducin 2 (beta); Adducin-2 (beta); beta adducin; Beta-adducin; Erythrocyte adducin subunit beta

Application:

WB IHC IF/ICC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500

	Western blot analysis of ADD1 phosphorylation expression in Forskolin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-ADD1 (Ser726) Antibody detects endogenous levels of ADD1 only when phosphorylated at Serine 726

Immunogen:

A synthesized peptide derived from human ADD1 around the phosphorylation site of Serine 726

Description:

ADD2 a cytoskeletal protein that promotes the assembly of the spectrin-actin network. Adducin is a heterodimeric protein that consists of related subunits. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.