Alternative Names:

AKT, AKT1 kinase, C-AKT, EC 2.7.11.1, PKB, PKB-alpha, Protein kinase B, RAC, RAC-PK-alpha, RAC-alpha serine/threonine kinase, kinase Akt1

Application:

WB

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000

	Western blot analysis on 293 cell lysate using Phospho-AKT1(Thr308) Antibody

Purification:

Affinity-chromatography

Specificity:

Phospho-AKT1(Thr308) Antibody detects endogenous levels of AKT1 only when phosphorylated at Threonine 308

Immunogen:

A synthesized peptide derived from human AKT1 around the phosphorylation site of Threonine 308

Description:

an AGC kinase that plays a critical role in controlling the balance between survival and AP0ptosis. Phosphorylated and activated by PDK1 in the PI3 kinase pathway. Mediates survival signals downstream of PI3 kinase and several growth factor receptors by phosphorylating AP0pototic proteins. First found in a mouse transforming retrovirus. Tumorigenic in a mouse lymphoma model and activated (by phospho-Akt staining) and/or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Inhibitor: RX-0201. Substrates include tuberin, Bad, Forkhead transcription factors, caspase-9, and glycogen synthase kinase-3.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.