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Details
Alternative Names:
Apoptosis signal- regulating kinase 1; ASK-1; EC 2.7.11.25; kinase ASK1; M3K5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5; MEK kinase 5; MEKK 5; MEKK5; Mitogen-activated protein kinase kinase kinase 5
Application:
WB IHC
Reactivity:
Human
Dilution:
WB 1:500-1:2000 IHC 1:50-1:200
Western blot analysis of ASK1 phosphorylation expression in TNF-α treated MDA-MB-435 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. | |
Purification:
Affinity-chromatography
Specificity:
Phospho-ASK1 (Ser83) Antibody detects endogenous levels of ASK1 only when phosphorylated at Serine 83
Immunogen:
A synthesized peptide derived from human ASK1 around the phosphorylation site of Serine 83
Description:
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK.
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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