Alternative Names:

Apopain precursor, CASP-3, CPP-32, CPP32, Caspase-3, Cysteine protease CPP32, EC 3.4.22.-, ICE3, SCA-1, SREBP cleavage activity 1, Yama protein

Application:

WB IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200

	Western blot analysis of Caspase 3 phosphorylation expression in Etoposide treated Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-Caspase 3 (Ser150) Antibody detects endogenous levels of Caspase 3 only when phosphorylated at Serine 150

Immunogen:

A synthesized peptide derived from human Caspase 3 around the phosphorylation site of Serine 150

Description:

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.