Alternative Names:

CDC25M3, Dual specificity phosphatase Cdc25A, EC 3.1.3.48, M-phase inducer phosphatase 1, MPIP1

Application:

WB IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200

	Western blot analysis of CDC25A phosphorylation expression in UV treated A549 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-CDC25A (Ser178) Antibody detects endogenous levels of CDC25A only when phosphorylated at Serine 178

Immunogen:

A synthesized peptide derived from human CDC25A around the phosphorylation site of Serine 178

Description:

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.