Alternative Names:

CDC25HU2, CDC25M2, Dual specificity phosphatase Cdc25B, EC 3.1.3.48, M-phase inducer phosphatase 2, MPIP2

Application:

WB

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000

	Western blot analysis of CDC25B phosphorylation expression in PMA treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-CDC25B (Ser323) Antibody detects endogenous levels of CDC25B only when phosphorylated at Serine 323

Immunogen:

A synthesized peptide derived from human CDC25B around the phosphorylation site of Serine 323

Description:

CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.