Alternative Names:

Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1; CHK1 checkpoint homolog (S. pombe); Serine/threonine-protein kinase Chk1

Application:

WB IF/ICC

Reactivity:

Human

Dilution:

WB 1:500-1:2000 IF/ICC 1:100-1:500

	Western blot analysis of Chk1 phosphorylation expression in Na3VO4 treated K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-Chk1 (Ser286) Antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 286

Immunogen:

A synthesized peptide derived from human Chk1 around the phosphorylation site of Serine 286

Description:

DNA damage induced protein phosphorylation; regulation of mitotic centrosome separation; regulation of S phase; peptidyl-threonine phosphorylation; DNA repair; chromatin-mediated maintenance of transcription; negative regulation of mitosis;

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.