Alternative Names:

1110020L01Rik; Amplaxin; cortactin; CTTN; EMS1; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate); FLJ34459; Oncogene EMS1; Src substrate cortactin; SRC8

Application:

WB

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000

	Western blot analysis of Cortactin phosphorylation expression in H2O2 treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-Cortactin (Tyr421) Antibody detects endogenous levels of Cortactin only when phosphorylated at Tyrosine 421

Immunogen:

A synthesized peptide derived from human Cortactin around the phosphorylation site of Tyrosine 421

Description:

cortactin a cytoskeletal protein that that is involved in coordinating actin reorganization during cell movement. Localizes at the leading edge of lamellipodia during cell migration. Its amino-terminal acidic domain associates with the Arp2/3 and WASP complex at F-actin branches.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.