Alternative Names:

EC 2.7.11.25 COT proto-oncogene serine/threonine-protein kinase C-COT Cancer Osaka thyroid oncogene Tumor progression locus 2 TPL-2

Application:

WB IHC IF/ICC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500

	Western blot analysis of COT phosphorylation expression in UV treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-COT (Thr290) Antibody detects endogenous levels of COT only when phosphorylated at Threonine 290

Immunogen:

A synthesized peptide derived from human COT around the phosphorylation site of Threonine 290

Description:

This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.