Alternative Names:

17 kDa PKC-potentiated inhibitory protein of PP1; 17-KDa protein; 17-kDa PKC-potentiated inhibitory protein of PP1; CPI-17; CPI17; PKC-potentiated inhibitory protein of PP1; PP14A; PPP1INL; PPP1R14A; Protein kinase C-potentiated inhibitor protein of 17 kD

Application:

WB IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200

	Western blot analysis of CPI17 alpha phosphorylation expression in RAW264.7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-CPI17 alpha (Thr38) Antibody detects endogenous levels of CPI17 alpha only when phosphorylated at Threonine 38

Immunogen:

A synthesized peptide derived from human CPI17 alpha around the phosphorylation site of Threonine 38

Description:

PPP1R14A a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.