Alternative Names:

C-RAF, C-Raf, CRAF, EC 2.7.11.1, Protein kinase raf 1, RAF proto-oncogene serine/threonine-protein kinase, RAF-1, RAF1, Raf proto-oncogene serine/threonine-protein kinase, Raf-1, Similar to murine leukemia viral (V-raf-1) oncogene homolog 1, kinase Raf1

Application:

WB

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000

	Western blot analysis on Jurkat cell lysate using Phospho-C-RAF(Ser301) Antibody

Purification:

Affinity-chromatography

Specificity:

Phospho-C-RAF(Ser301) Antibody detects endogenous levels of C-RAF only when phosphorylated at Sersine 301

Immunogen:

A synthesized peptide derived from human C-RAF around the phosphorylation site of Sersine 301

Description:

Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.