Alternative Names:

E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; RBBP3; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Transcription factor E2F1

Application:

WB

Reactivity:

Human, Mouse

Dilution:

WB 1:500-1:2000

	Western blot analysis of E2F1 phosphorylation expression in Etoposide treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-E2F1 (Thr433) Antibody detects endogenous levels of E2F1 only when phosphorylated at Threonine 433

Immunogen:

A synthesized peptide derived from human E2F1 around the phosphorylation site of Threonine 433

Description:

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.