Alternative Names:

EIF-2; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2; EIF2A; EIF2S1; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; IF2A

Application:

WB

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000

	Western blot analysis of eIF2 alpha phosphorylation expression in IFN-α treated K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-eIF2 alpha (Ser51) Antibody detects endogenous levels of eIF2 alpha only when phosphorylated at Serine 51

Immunogen:

A synthesized peptide derived from human eIF2 alpha around the phosphorylation site of Serine 51

Description:

eIF2A a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.