Alternative Names:

eIF-4B; Eukaryotic translation initiation factor 4B; IF4B

Application:

WB,IHC,IF

Reactivity:

Human,Mouse

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200 IF 1:100-1:500

	Western blot analysis of eIF4B (Phospho-Ser422) expression in HepG2 cells

Purification:

Affinity-chromatography

Specificity:

eIF4B (Phospho-Ser422) Antibody detects endogenous levels of total eIF4B (Phospho-Ser422)

Immunogen:

A synthesized peptide derived from human eIF4B (Phospho-Ser422)

Description:

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.