Alternative Names:

ACLS; GCPS; GLI3; GLI3FL; PAPA; PAPA1; PAPB; PHS; PPDIV;

Application:

WB ICC/IF

Reactivity:

Human

Dilution:

WB 1:500~1:2000 ICC/IF 1:50~1:200

	Western blot analysis of Gli3 expression in 293 cell lysate.

Purification:

Affinity-chromatography

Specificity:

Gli3 Antibody detects endogenous levels of total Gli3

Immunogen:

A synthesized peptide derived from human Gli3

Description:

Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.