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pGAD424
pGAD424
Product Introduction

pGAD424 

基本信息

质粒类型: 酵母单杂交系统
启动子: ADH1
载体大小: 6659 bp (查看载体序列)
载体抗性: Ampicillin (氨苄青霉素)

订购信息

产品编号 产品名称 规格 价格
YB1644 pGAD424

5ug质粒

¥1000.00

质粒图谱

pGAD424 质粒图谱

载体描述

pGAD424 generates a hybrid protein that contains the sequences for the GAL4 activation domain (AD; a.a. 768–881) (1). pGAD424 has unique restriction sites located in the MCS region at the 3'- end of the open reading frame for the GAL4 AD sequence. For the construction of a hybrid protein, the gene encoding the protein of interest (or a collection of cDNAs) is ligated into the MCS in the correct orientation and in the correct reading frame such that a fusion protein is generated. The fusion protein is expressed at high levels in yeast host cells from the constitutive ADH1 promoter; transcription is terminated at the ADH1 transcription termination signal. The hybrid protein is targeted to the yeast nucleus by nuclear localization sequences that have been added to the AD sequence from a heterologous source (2). pGAD424 is a shuttle vector that replicates autonomously in both E. coli and S. cerevisiae. It carries the bla gene (for ampicillin resistance in E. coli) and the LEU2 nutritional marker that allow yeast auxotrophs carrying pGAD424 to grow on limiting synthetic medium lacking Leu.


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