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pIEX/Bac-1
质粒类型: | 昆虫细胞表达系统 |
---|---|
启动子: | IE1, p10 |
表达水平: | 高 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 6796 bp (查看载体序列) |
载体标签: | C-His,N-Strep |
载体抗性: | Ampicillin (氨苄青霉素) |
备注: | 拥有hr5增强子 |
The dual-purpose pIEx/Bac™ vectors are designed for cloning and high-level expression of proteins by transiently transfecting Spodoptera-derived insect cells or by generating baculovirus recombinants. Transient transfection and early baculovirus expression is driven by a promoter/enhancer combination that recruits endogenous insect cell transcription machinery, the AcNPV derived hr5 enhancer and ie1 promoter. Late/very late expression in the baculovirus mode is driven by the strong p10 promoter. The pIEx/Bac-1 vector carries an N-terminal Strep•Tag II coding sequence (1) followed by a recognition site for enterokinase.
The multiple cloning region is followed by an optional C-terminal His•Tag® coding sequence. The presence of two “gentle elution" tags at both the N- and C-terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins (2). Unique restriction sites are shown on the circle map.
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