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pRNATin-H1.2/Neo
| 质粒类型: | RNAi载体 |
|---|---|
| 启动子: | H1.2 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6127 bp (查看载体序列) |
| 5' 测序引物及序列: | TAATACGACTCACTATAGGG |
| 3' 测序引物及序列: | TAGAAGGCACAGTCGAGG |
| 载体标签: | cGFP |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 筛选标记: | Neomycin (新霉素) |
pRNATin-H1.2/Neo/siFluc is a GenScript siRNA expression vector. It is designed for mammalian transfection. The GFP marker (coral GFP, cGFP) under CMV promoter control can be used to track the transfection efficiency. It uses the H1.2 promoter, an engineered inducible promoter containing a tetracycline operator (TetO1) for siRNA expression. This vector contains siRNA construct for Firefly luciferase, and can be used as a positive control. It can also be used as a siRNA vector using BamH I and Hind III for siRNA insertion.
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