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pAd/CMV/V5-DEST
| 质粒类型: | 腺病毒载体 |
|---|---|
| 启动子: | CMV |
| 克隆方法: | Gateway |
| 载体大小: | 36686 bp (查看载体序列) |
| 5' 测序引物及序列: | CMVPro Fwd: 5'd[CGCAAATGGGCGGTAGGCGTG]3' |
| 载体标签: | V5 |
| 载体抗性: | Ampicillin (氨苄青霉素) |
The pAd⁄CMV⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ adenoviral expression vector, pAd⁄CMV⁄V5-DEST™ vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where constitutive, high-level expression is driven by the CMV promoter.
Advantages
• High efficiency and rapid recombination cloning
• Produces high titer adenoviral stocks
• Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo
• Produces replication-incompetent virus for enhanced biosafety of the system
• Amenable for use in high-throughput applications
Key Features
• Gateway® Technology for efficient and rapid cloning
• CMV promoter for high-level constitutive expression of gene of interest
• Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the
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