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pFLAG-CMV-2
| 质粒类型: | 哺乳动物表达载体 |
|---|---|
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4679 bp (查看载体序列) |
| 5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG ; hGH-pA-R: CCAGCTTGGTTCCCAATAGA |
| 载体标签: | FLAG (N terminal) |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 备注: | shuttle vector for intracellular transient expression of N-terminal Met-FLAG |
The pFLAG-CMV-2 Expression Vector is a 4.7 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG fusion proteins in mammalian cells.
The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG® -fusion constructs.
pFLAG-CMV-2 Expression Vector is a shuttle vector for E. coli and mammalian cells. SV40 replication origin results in most efficient replication in COS cells. The pFLAG-CMV-2-BAP Control Plasmid is a 6.1 kb derivative of the pCMV5 transient expression vector1 for intracellular expression of N-terminal Met-FLAG bacterial alkaline phosphatase fusion protein in mammalian cells.
The promoter-regulatory region of the human cytomegalovirus2 drives transcription of FLAG-BAP constructs.
pFLAG-CMV-2-BAP Control Plasmid is a shuttle vector for E. coli and mammalian cells. SV40 replication origin results in most efficient replication in COS cells.
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