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pcDNA-DEST40
Product Introduction
pcDNA-DEST40
| 质粒类型: | Gateway载体 |
|---|---|
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 7143bp (查看载体序列) |
| 5' 测序引物及序列: | T7 Fwd: 5'd[TAATACGACTCACTATAGGG]3' |
| 载体标签: | V5 |
| 载体抗性: | Ampicillin (氨苄青霉素) |
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The Gateway® pcDNA™-DEST40 vector offers the following key features:
- C-terminal V5-6x His tag for easy detection and rapid purification
- Cytomegalovirus (CMV) promoter for high-level expression
- attR sites for Gateway® cloning, enabling recombination with attL-flanked fragments
- Neomycin resistance gene for stable selection
- Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
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