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pAc5.1/V5-His C
| 别名: | pAc5.1/V5-HisC |
|---|---|
| 质粒类型: | 果蝇细胞表达载体 |
| 启动子: | pAC5 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5371 bp (查看载体序列) |
| 5' 测序引物及序列: | AC5:5'd[ACACAAAGCCGCTCCATCAG]3' |
| 载体标签: | V5 Epitope Tag, His Tag (6x) |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 备注: |
Constitutive insect cell expression. Can use pCoHygro or pCoBlast for selection.
|
The DES®-Constitutive Kit provides the vector pAc5.1/V5-His for high-level constitutive expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. The vector offers the strong, constitutive promoter from the Drosophila actin 5C gene. The pAc5.1/V5-His vector also offers the following features:
- Small size (5.4 kb) to improve DNA yields and increase subcloning efficiency
- C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
- C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.
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