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pIZT/V5-His
| 质粒类型: | 昆虫细胞表达载体 |
|---|---|
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | OpIE2 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3336bp (查看载体序列) |
| 5' 测序引物及序列: | OpIE2：5'd[CGCAACGATCTGGTAAACAC]3' |
| 载体标签: | V5 (Cterm)， His (Cterm) |
| 载体抗性: | Zeocin (博莱霉素) |
| 筛选标记: | Zeocin |
| 备注: | Stable expression of gene in insect cells. GFP reporter allows visualization of stable cells. |
载体描述
The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of yourgene of interest. This vector has the following features:
- The OpIE2 promoter for constitutive expression
- The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
- C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistanceprotein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lineswith Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.
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