Recombinant Human Insulin-like Growth factor-1 GMP

Synonyms Somatomedin C, IGF-I, IGF-IA

Accession P05019

GeneID 3479

Source Escherichia coli.

Molecular Weight Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.

Quantity 5µg/100µg/1mg

AA Sequence GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Purity > 98 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.

Endotoxin Less than 0.01 EU/μg of rHuIGF-1 GMP as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.

1 month, 2 to 8 °C under sterile conditions after reconstitution.

3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This GMP product can be for research use or further manufacturing use.

Quality statement The manufacture and testing of this product is in compliance with ICH Q7a guidelines.

Reference 1. Skottner A, Fryklund L, Hansson HA. 1986. Acta Paediatr Scand Suppl, 325: 107-11.

2. Bartlett WP, Li XS, Williams M. 1992. Brain Res Mol Brain Res, 12: 285-91.

3. Palmade F, Sechoy-Chambon O, Coquelet C, et al. 1994. Curr Eye Res, 13: 531-7.

4. Tennagels N, Hube-Magg C, Wirth A, et al. 1999. Biochem Biophys Res Commun, 260: 724-8.

5. Laron Z. 2004. Novartis Found Symp, 262: 56-77; discussion -83, 265-8.

6. Shiratsuchi I, Akagi Y, Kawahara A, et al. 2011. Anticancer Res, 31: 2541-5.

Background The insulin-like growth factors (IGFs) belonged to the insulin gene family, are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Recombinant human IGF-1 are globular proteins containing 70 amino acids and 3 intra-molecular disulfide bonds. Mature human IGF-1 shares 94 % and 96 % a.a. sequence identity with mouse and rat IGF-1, respectively, and exhibits cross-species activity.