Recombinant Streptavidin

Accession P22629

Source Escherichia coli.

Molecular Weight ~52,000 per tetramer.

AA Sequence MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS

A282 of 0.1% solution 3.1

Purity > 98 % by SDS-PAGE and HPLC analyses

Specific Activity > 17 U/mg (one unit binds 1 µg D-biotin)

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized in 10 mM potassium phosphate buffer pH 6.5.

Endotoxin Less than 0.1 EU/μg of rStreptavidin as determined by LAL method.

Proteolytic Activity < 10-3 U/mg protein (Azocoll, 25 °C, 24 h, pH 8.0)

Reconstitution Dissolve with double distilled water

Solubility Clearly soluble in ddH2O (OD405nm < 0.1 at 10 mg/ml water)

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Shipment At ambient temperature

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