Recombinant Murine Interleukin-4

Synonyms B-cell IgG differentiation factor, B-cell growth factor 1, BSF-1, IGG1 induction factor, Lymphocyte stimulatory factor 1

Accession P07750

GeneID 16189

Source Escherichia coli.

Molecular Weight Approximately 13.5 kDa, a single non-glycosylated polypeptide chain containing 121 amino acids.

Quantity 5µg/20µg/1mg

AA Sequence MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant prolifiration of Murine HT-2 cells is less then 2 ng/ml, corresponding to a Specific Activity of > 5 × 105 IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/µg of rMuIL-4 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Sokol CL, Barton GM, Farr AG, et al. 2008. Nat Immunol. 9:310-8.

2. Hershey GK, Friedrich MF, Esswein LA, et al. 1997. N Engl J Med. 337:1720-5.

3. Yokota T, Otsuka T, Mosmann T, et al. 1986. Proc Natl Acad Sci U S A. 83:5894-8.

4. Eder A, Krafft-Czepa H, Krammer PH. 1988. Nucleic Acids Res. 16:772.

5. Walter MR, Cook WJ, Zhao BG, et al. 1992. J Biol Chem. 267:20371-6

Background Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. It is produced by mast cells, activated T cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. In addition, IL-4 enhances both secretion and cell surface expression of IgE and IgG1 and also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The mouse IL-4 has a compact, globular fold, stabilised by 3 disulphide bonds and contains 121 amino acids residues which is a single non-glycosylated polypeptide. The human IL-4 shares about 40% aa sequence identity with mouse/rat IL-4 and they are species-specific in their activities.