Protein A/G/L-Cys

Source Escherichia coli.

Molecular Weight Approximately 89.3 kDa with a Cys on C-terminus, a single non-glycosylated polypeptide chain containing 806 amino acids.

AA Sequence NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDSLEG SGSGTYKLIL NGKTLKGETT TEAVDAATAE KVFKQYANDN GVDGEWTYDD ATKTFTVTEK PEVIDASELT PAVTTYKLVI NGKTLKGETT TKAVDAETAE KAFKQYANDN GVDGVWTYDD ATKTFTVTEE PRARPGSGSG KEETPETPET DSEEEVTIKA NLIFANGSTQ TAEFKGTFEK ATSEAYAYAD TLKKDNGEYT VDVADKGYTL NIKFAGKEKT PEEPKEEVTI KANLIYADGK TQTAEFKGTF EEATAEAYRY ADALKKDNGE YTVDVADKGY TLNIKFAGKE KTPEEPKEEV TIKANLIYAD GKTQTAEFKG TFEEATAEAY RYADLLAKEN GKYTVDVADK GYTLNIKFAG KEKTPEEPKE EVTIKANLIY ADGKTQTAEF KGTFAEATAE AYRYADLLAK ENGKYTADLE DGGYTINIRF AGKKVDEKPE EKEQVTIKEN IYFEDGTVQT ATFKGTFAEA TAEAYRYADL LSKEHGKYTA DLEDGGYTIN IRFAGC

Protein per Gram Powder See Label.

Concentration for 1 unity DO at 280 nm 1.412 mg/ml

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Purity > 95 % by SDS-PAGE and SEC-HPLC analyses.

Specificity The recombinant Protein A/G/L is a genetically engineered protein containing 5 IgG-binding regions of protein A, 2 of protein G and 5 of Protein L. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A/G/L to ensure the maximum specific IgG binding. The recombinant Protein A/G/L is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A/G/L binds to human, mouse, rat cow, goat, sheep, rabbit, guinea pig, pig, dog and cat IgG.

Endotoxin Level Less than 0.1 EU/μg of Protein A/G/L-Cys as determined by LAL method.

Formulation Lyophilized from additive free solution.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Dissolve in distilled water or saline.

Background Protein A/G/L is a genetically engineered protein that combines the IgG binding profiles of all Protein A, Protein G and Protein L. It is a gene fusion product. Recombinant fusion protein A/G/L contains five Ig-binding regions of protein L, five IgG binding domains from Protein A, and two Ig-binding region of protein G. Cell wall binding regions, albumin binding regions and other non-specific binding regions have all been eliminated from the fusion protein to ensure the maximum specific IgG binding.