Protein A-Cys, His

Accession P02976

GeneID 3919448

Source Escherichia coli.

Molecular Weight Approximately 34.8 kDa with 6×His-tag and a Cys on C-terminus, a single non-glycosylated polypeptide chain containing 307 amino acids.

AA Sequence MNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE HHHHHHC

Protein per Gram Powder See Label.

Concentration for 1 unity DO at 280 nm 4.589 mg/ml

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Purity > 96 % by SDS-PAGE and RP-HPLC analyses.

Specificity The recombinant Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.

Endotoxin Level Less than 0.1 EU/μg of Protein A-Cys, His as determined by LAL method.

Formulation Lyophilized from additive free solution.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Dissolve in distilled water or saline.

Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at -20 °C.

Background Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E.Coli and functions essentially the same as native Protein A. It consists of 5 IgG-binding domains E-D-A-B-C aligned in series.