Recombinant Rat Interleukin-2, Liquid

Synonyms T-cell Growth Factor, CGF

Accession P17108

GeneID 116562

Source Escherichia coli.

Molecular Weight Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 135 amino acids.

Quantity 5µg/20µg/1mg

AA Sequence APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.

Physical Appearance Sterile Colorless liquid.

Formulation A 0.2 μm filtered concentrated sterile solution in 10 mM sodium citrate, pH 4.0, 30 % glycerol.

Endotoxin Less than 1 EU/μg of rRtIL-2, Liquid as determined by LAL method.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 6 months from date of receipt, -20 to -70 °C as supplied.

- 3 months, -20 to -70 °C under sterile conditions after opening.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Ma, A., R. Koka, and P. Burkett. 2006. Annu Rev Immunol, 24: 657-79.

2. Taniguchi, T., H. Matsui, T. Fujita, et al. 1983. Nature, 302: 305-10.

3. Liparoto, S.F., D.G. Myszka, Z. Wu, et al. 2002. Biochemistry, 41: 2543-51.

4. Bodnar, A., E. Nizsaloczki, G. Mocsar, et al. 2008. Immunol Lett, 116: 117-25.

5. Mosmann, T.R., T. Yokota, R. Kastelein, et al. 1987. J Immunol, 138: 1813-6.

Background IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues and it shares about 82 %, 66 %, 66 %, 60 % and 54 % amino acid sequence identity with mouse, human, rhesus macaque, pig and equus caballus IL-2, respectively.