Recombinant Human Thymosin beta 4

Synonyms Fx

Accession P62328

GeneID 7114

Source Escherichia coli.

Molecular Weight Approximately 4.9 kDa, a single non-glycosylated polypeptide chain containing 43 amino acids.

Quantity 20µg/100µg/1mg

AA Sequence SDKPDMAEIE KFDKSKLKKT ETQEKNPLPS KETIEQEKQA GES

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity determined by its ability to produce a protective effect against hydrogen peroxide in primary lung fibroblasts is in a concentration range of 0.5 - 10 μg/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4.

Endotoxin Less than 1 EU/µg of rHuTβ4 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Moon EY, Song JH, Yang KH. 2007. Oncol Res, 16: 507-16.

2. Oh SY, Song JH, Gil JE, et al. 2006. Exp Cell Res, 312: 1651-7.

3. Oh JM, Ryoo IJ, Yang Y, et al. 2008. Cancer Lett, 264: 29-35.

Background Thymosin Beta 4 is a naturally occurring peptide encoded by the TMSB4X gene located on Chr. X in humans. It is found in high concentrations in blood platelets, wound fluid and other tissues in the body. Tβ-4 is a major actin regulating peptide and the primary function is to stimulate the productions of T cells, which plays important part of the immune system. The thymosin beta-4 peptide, if used after a heart attack, might reactivate cardiac progenitor cells to repair damaged heart tissue.