Recombinant Human Interleukin-16, 130 a.a.

技术参数

Synonyms Interleukin-16 isoform 2, Lymphocyte chemoattractant factor, LCF

Accession Q14005

GeneID 3603

Source Escherichia coli.

Molecular Weight Approximately 13.4 kDa, a single non-glycosylated polypeptide chain containing 130amino acids.

Quantity 2µg/10µg/1mg

AA Sequence MPDLNSSTDS AASASAASDV SVESTAEATV CTVTLEKMSA GLGFSLEGGK GSLHGDKPLT INRIFKGAAS EQSETVQPGD EILQLGGTAM QGLTRFEAWN IIKALPDGPV TIVIRRKSLQ SKETTAAGDS

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CD4+ T Lymphocytes is in a concentration range of 1.0-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, 150 mM NaCl, pH 7.0.

Endotoxin Less than 1 EU/µg of rHuIL-16, 130 a.a. as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Baier M, Bannert N, Werner A, et al. 1997. Proc Natl Acad Sci U S A, 94: 5273-7.

2. Zhang Y, Center DM, Wu DM, et al. 1998. J Biol Chem, 273: 1144-9.

3. Cruikshank WW, Kornfeld H, Center DM. 2000. J Leukoc Biol, 67: 757-66.

4. Cruikshank WW, Center DM, Nisar N, et al. 1994. Proc Natl Acad Sci U S A, 91: 5109-13.

5. Mathy NL, Scheuer W, Lanzendorfer M, et al. 2000. Immunology, 100: 63-9.

Background Human Interleukin-16 (IL-16) is also called lymphocyte chemoattractant factor (LCF) and it has three isoforms which are cleaved from pre-Interleukin-16. IL-16 is mostly secreted by lymphocytes, epithelial cells, eosinophils, and CD8+ T cells. It has many functions including induction of the IL-2Rα on T cells, suppression of human immunodeficiency virus (HIV) replication, inhibition of T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions and so on. It signals through CD4 receptor. Furthermore, recombinant human IL-16 (130 a.a.) contains 130 amino acid residues and it shares 85 % a.a. sequence identity with murine IL-16.