Recombinant Murine Betacellulin

Accession Q05928

GeneID 12223

Source Escherichia coli.

Molecular Weight Recombinant human Betacellulin is a 9.0 kDa monomeric protein, containing 80 amino residues, which comprises the mature EGF homologous portion of the Betacellulin protein.

Quantity 5µg/20µg/1mg

AA Sequence DGNTTRTPET NGSLCGAPGE NCTGTTPRQK VKTHFSRCPK QYKHYCIHGR CRFVVDEQTP SCICEKGYFG ARCERVDLFY

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.01 ng/ml, corresponding to a specific activity of > 1.0 × 108 IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4.

Endotoxin Less than 1 EU/µg of rMuBetacellulin as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Miura K, Doura H, Aizawa T, et al. 2002. Biochem Biophys Res Commun. 294:1040-6.

2. Kim HS, Shin HS, Kwak HJ, et al. 2003. FASEB J. 17:318-20.

3. Nakagawa T, Furuta H, Sanke T, et al. 2005. Diabetes Res Clin Pract. 68:188-92.

Background Betacellulin (BTC) encoded by the BTC gene located on the chromosome 4, is a member of the EGF family of cytokines that also includes EGF, TGF-alpha, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulins. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids, including serum, milk, and colostrum. Mouse BTC is expressed as a 178-amino acid precursor and the amino acid sequence of the mature form is 80% identical with human BTC. Both human and mouse BTC exhibit significant overall similarity with other members of the EGF family. Recombinant murine beta-cellulin is a heparinbinding protein containing 80 amino acids residues.