Recombinant Bovine Enterokinase Light Chain, Yeast

Synonyms Enterokinase, Serine Protease 7, Transmembrane Protease Serine 15

Source Pichia Pastoris

Molecular Weight Approximately 43.0 kDa, a single glycosylated polypeptide chain containing 235 amino acids.

Quantity 100IU/250IU/1000IU

Unit Definition One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95 % completion at 25 °C in a buffer containing 25 mM Tris-HCl, pH 7.6, 50 mM NaCl, and 2 mM CaCl2.

Physical Appearance Sterile liquid.

Formulation 50 mM Tris-HCl, pH 8.0, 0.5 M NaCl and 50 % glycerol.

Endotoxin Less than 1 EU/µg of rBoEKL as determined by LAL method.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 6 months from date of receipt, -20 to -70 °C as supplied.

- 3 months, -20 to -70 °C under sterile conditions after opening.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Yuan LDandHua ZC. 2002. Protein Expr Purif, 25: 300-4.

2. Peng L, Zhong X, Ou J, et al. 2004. J Biotechnol, 108: 185-92.

3. Light AandJanska H. 1991. J Protein Chem, 10: 475-80.

4. Kubitzki T, Minor D, Mackfeld U, et al. 2009. Biotechnol J, 4: 1610-8.

Background Enterokinase (EK) is an amino protease existing in duodenum of mammal and is involved in digestion. It consists of a disulfide-linked 82–140 kDa heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35–62 kDa light chain which contains the catalytic subunit. Additionally, both of the chains are derived from a single precursor that is cleaved by a trypsin-like protease. EK can specially recognize the amino acid sequence DDDDK, and digest the peptide bond after the lysine residue. rEK was report to be more effective than nature EK in cleaving recombinant proteins. Furthermore, the light chain possesses the whole enzyme activity of EK. rBoEK has the highest activity than EK of other species and is used wildly in biochemical applications.