Recombinant Rat C10/CCL6

Synonyms Small-inducible Cytokine A6

Accession Q68FP3

GeneID 287910

Source Escherichia coli.

Molecular Weight Approximately 10.4 kDa, a single non-glycosylated polypeptide chain containing 94 amino acids.

Quantity 2µg/10µg/1mg

AA Sequence GLIQDTVKED RPFNPTIIHQ GFQDSSDCCF SYASQIPCSR FIYYFPTSGG CTKPGIIFVT RKRKRVCANP SDQRVQTCIS TLKLGPRSGN SAIA

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CCR1 transfected murine BaF3 cells is in a concentration range of 10-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.

Endotoxin Less than 1 EU/μg of rRtC10/CCL6 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Berger MS, Kozak CA, Gabriel A, et al. 1993. DNA Cell Biol. 12:839-47.

2. Orlofsky A, Berger MS, Prystowsky MB. 1991. Cell Regul. 2:403-12.

3. Ma B, Zhu Z, Homer RJ, et al. 2004. J Immunol. 172:1872-81.

Background Chemokine (C-C motif) ligand 6 (CCL6), encoded by the gene CCL6, is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. It signals primarily through the CCR1 receptor. CCL6 is chemotactic for B cells, CD4+ T cells, monocytes and NK cells and also exhibits powerful suppressive activity on colony formation by different lineages of hematopoietic progenitors. It contains the four highly conserved cysteine residues present in CC chemokines.