Recombinant Human Trefoil Factor 2

Synonyms Spasmolysin, Spasmolytic polypeptide, SP

Accession Q03403

GeneID 7032

Source Escherichia coli.

Molecular Weight Approximately 12.0 kDa, a single non-glycosylated polypeptide chain containing 106 amino acids, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.

Quantity 5µg/20µg/1mg

AA Sequence EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKS VEDCHY

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.

Endotoxin Less than 1 EU/µg of rHuTFF2 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Seib T, Blin N, Hilgert K, et al. 1997. Genomics, 40: 200-2.

2. Kirikoshi HandKatoh M. 2002. Int J Oncol, 21: 655-9.

3. Xue L, Aihara E, Podolsky DK, et al. 2010. Gut, 59: 1184-91.

4. Poulsen SS, Thulesen J, Christensen L, et al. 1999. Gut, 45: 516-22.

Background Trefoil factor 2 encoded by the TFF2 gene in humans, belongs to the trefoil factor family that consists of three members named TFF1, TFF2 and TFF3. They are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. The TFFs are stable secretory proteins expressed highly in the gastrointestinal tract (gastric mucosa). TFF2 can inhibit gastrointestinal motility and gastric acid secretion. Additionally, it functions as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.