Recombinant Murine Dendritic Cell Inflammatory Protein-1/CXCL3

Accession Q6W5C0

GeneID 330122

Source Escherichia coli.

Molecular Weight Approximately 7.9 kDa, a single, non-glycosylated polypeptide chain containing 73 amino acids.

Quantity 2µg/10µg/1mg

AA Sequence AVVASELRCQ CLNTLPRVDF ETIQSLTVTP PGPHCTQTEV IATLKDGQEV CLNPQGPRLQ IIIKKILKSG KSS

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rMuDCIP-1/CXCL3 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Nolan KF, Strong V, Soler D, et al. 2004. J Immunol. 172:2201-9.

2. Modi WS, Yoshimura T. 1999. Mol Biol Evol. 16:180-93.

3. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.

4. Smith DF, Galkina E, Ley K, et al. 2005. Am J Physiol Heart Circ Physiol. 289:H1976-84.

5. Wuyts A, Govaerts C, Struyf S, et al. 1999. Eur J Biochem. 260:421-9.

Background CXCL3, also known as DCIP1 in murine, CINC2 in rat, and GROγ in humans, is belonging to the CXC chemokine family. It is encoded by the gene CXCL3 in mouse. The functional receptor for CXCL3 has been identified as CXCR2. Similar to other GRO proteins, CXCL3 is potent neutrophil attractants and activators. CXCL3 plays a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. The amino acid sequence of murine CXCL3 is 57 % identical to human CXCL3.