Recombinant Murine Monokine Induced by Interferon-gamma/CXCL9

Synonyms Gamma-interferon-induced Monokine, Small-inducible Cytokine B9

Accession P18340

GeneID 17329

Source Escherichia coli.

Molecular Weight Approximately 12.2 kDa, a single non-glycosylated polypeptide chain containing 105 amino acids.

Quantity 5µg/20µg/1mg

AA Sequence TLVIRNARCS CISTSRGTIH YKSLKDLKQF APSPNCNKTE IIATLKNGDQ TCLDPDSANV KKLMKEWEKK INQKKKQKRG KKHQKNMKNR KPKTPQSRRR SRKTT

Purity > 95 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 0.1-1.0 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4.

Endotoxin Less than 1 EU/µg of rMuMIG/CXCL9 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Lee HH, Farber JM. 1996. Cytogenet Cell Genet. 74:255-8.

2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.

3. Tensen CP, Flier J, Van Der Raaij-Helmer EM, et al. 1999. J Invest Dermatol. 112:716-22.

4. Adamska I, Roobol-Boza M, Lindahl M, et al. 1999. Eur J Biochem. 260:453-60.

Background CXCL9 belongs to the CXC chemokine family and also known as Monokine induced by gamma interferon (MIG). It is a T-cell chemoattractant induced by IFN-γ. CXCL9 is closely related to two other CXC chemokines called CXCL10 and CXCL11, additionally they all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. It is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response and chemotactic for activated T-cells. Recombinant murine CXCL9 contains 105 amino acids which is a single non-glycosylated polypeptide chain. Furthermore, The murine CXCL9 shares 75% and 88% a.a. sequence identity with human and rat CXCL9.