Recombinant Murine gamma-Interferon Inducible Protein 10/CXCL10

Synonyms Gamma-IP10, Small-inducible Cytokine B10, C7, Interferon-gamma Induced Protein CRG-2

Accession P17515

GeneID 15945

Source Escherichia coli.

Molecular Weight Approximately 8.7 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids.

Quantity 5µg/25µg/1mg

AA Sequence IPLARTVRCN CIHIDDGPVR MRAIGKLEII PASLSCPRVE IIATMKKNDE QRCLNPESKT IKNLMKAFSQ KRSKRAP

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human peripheral blood lymphocytes is in a concentration range of 0.1-10.0 ng/ml in the presence of IL-2.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4.

Endotoxin Less than 1 EU/µg of rMuIP-10/CXCL10 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Luster AD, Unkeless JC, Ravetch JV. 1985. Nature. 315:672-6.

2. Luster AD, Jhanwar SC, Chaganti RS, et al. 1987. Proc Natl Acad Sci U S A. 84:2868-71.

3. Vanguri P, Farber JM. 1990. J Biol Chem. 265:15049-57.

4. Booth V, Keizer DW, Kamphuis MB, et al. 2002. Biochemistry. 41:10418-25.

5. Dufour JH, Dziejman M, Liu MT, et al. 2002. J Immunol. 168:3195-204.

6. Angiolillo AL, Sgadari C, Taub DD, et al. 1995. J Exp Med. 182:155-62.

Background CXCL10 also known as IP-10 is belonging to the CXC chemokine family. It is encoded by the CXCL10 gene, and in murine it is also named the CRG-2 gene. The gene was originally identified as an immediate early gene induced in response to macrophage activation. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes and monocytes/macrophages. It also has other roles, such aspromotion of T cell adhesion to endothelial cells, and inhibition of bone marrow colony formation and angiogenesis. Murine CXCL10 shares approximately 67 % amino acid sequence identity with human CXCL10.