Recombinant Human BCA-1/CXCL13

Synonyms Angie, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13

Accession O43927

GeneID 10563

Source Escherichia coli.

Molecular Weight Approximately 10.3 kDa, a single non-glycosylated polypeptide chain containing 87 amino acids.

Quantity 5µg/20µg/1mg

AA Sequence VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human B cells is in a concentration range of 1.0-10 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.

Endotoxin Less than 1 EU/μg of rHuBCA-1/CXCL13 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Reference 1. Legler DF, Loetscher M, Roos RS, et al. 1998. J Exp Med. 187:655-60.

2. Gunn MD, Ngo VN, Ansel KM, et al. 1998. Nature. 391:799-803.

3. Ansel KM, Harris RB, Cyster JG. 2002. Immunity. 16:67-76.

4. Ansel KM, Ngo VN, Hyman PL, et al. 2000. Nature. 406:309-14.

Background C-X-C motif chemokine 13 encoded by the CXCL13 gene located on human chromosome 4 is constitutively expressed in secondary lymphoid organs. It is also known as B lymphocyte chemoattractant (BLC). As its name suggests, CXCL13 is selectively chemotactic for B cells belonging to both the B-1 and B-2 subsets, and elicits its effects by interacting with chemokine receptor CXCR5. It and its receptor CXCR5 control the organization of B cells within follicles of lymphoid tissues. Recombinant human CXCL13 is a single non-glycosylated polypeptide chain containing 87 amino acids and mature human BCA-1 shares 64 % amino acid sequence similarity with the murine protein and 23–34 % amino acid sequence identity with other known CXC chemokines.