Recombinant Murine Mucosae-associated Epithelial Chemokine/CCL28

Synonyms CCK1

Accession Q9JIL2

GeneID 56838

Source Escherichia coli.

Molecular Weight Approximately 12.6 kDa, a single, non-glycosylated polypeptide chain containing 111 amino acids.

Quantity 5µg/20µg/1mg

AA Sequence SEAILPMASS CCTEVSHHVS GRLLERVSSC SIQRADGDCD LAAVILHVKR RRICISPHNR TLKQWMRASE VKKNGRENVC SGKKQPSRKD RKGHTTRKHR TRGTHRHEAS R

Purity > 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine lymphocytes is in a concentration range of 1.0-10.0 ng/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Endotoxin Less than 1 EU/μg of rMuMEC/CCL28 as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

- 12 months from date of receipt, -20 to -70 °C as supplied.

- 1 month, 2 to 8 °C under sterile conditions after reconstitution.

- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

SDS-PAGE

Reference 1. Wang W, Soto H, Oldham ER, et al. 2000. J Biol Chem, 275: 22313-23.

2. Hieshima K, Ohtani H, Shibano M, et al. 2003. J Immunol, 170: 1452-61.

3. Eksteen B, Miles A, Curbishley SM, et al. 2006. J Immunol, 177: 593-603.

4. Kagami S, Kakinuma T, Saeki H, et al. 2005. J Invest Dermatol, 124: 1088-90.

Background CCL28 belongs to CC family chemokine, also named mucosae-associated epithelial chemokine (MEC). The CCL28 mRNA is highly expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. The amino acids sequence of human and mouse CCL28 shares 83% identity, when the protein is processed to be the mature form. CCL28 is signaling through CCR3 and CCR10, and chemotactic for resting CD4, CD8 T-cells and eosinophils.